林华庆,余楚钦,邱五龙
LIN Hua-qing~1,YU Chu-qin~1,QIU Wu-long~2 (1.Institue of Materia Medica

• 1:广东药学院药物研究所
• 2:广东药学院药物研究所
• 3:广州固志医药科技有限公司 广州510224
• 4:广州510224

摘要(Abstract)：

目的制定和胃胶囊中延胡索乙素的含量测定方法,以更好的控制产品质量。方法采用HPLC进行测定,色谱柱为Hypersil BDS C18(5μm,4.6 mm×150 mm);流动相为甲醇-0.1%磷酸(用三乙胺调节pH至6.0)(60∶40);检测波长为280nm。结果和胃胶囊中延胡索乙素的线性范围为0.104~0.728μg,r=0.9997,平均回收率为98.95%。结论该方法操作简单、重现性好、专属性高,可作为和胃胶囊含量测定的指标。
Objective To establish the determination method of Tetrahydropalmatine in Hewei Jiaonang.Method The HPLC method for Tetrahydropalmatine was used.Column:Hypersil BDS-C_(18)(5 μm,4.6 mm×150 mm),mobile phase:methanol:0.1% H_3PO_4(60∶40).It was detected at 280 nm.Result The linear range of Tetrahydropalmatine was 0.104～0.728 μg,r=0.9997,The mean recovery was 98.95%.Conclusion This method is simple and accurate with good reproducibility.The method could be applied to determine Tetrahydropalmatine in Hewei Jiaonang.

关键词(KeyWords)： 和胃胶囊;延胡索乙素;HPLC
Hewei Jiaonang;tetrahydropalmatine;HPLC

Abstract:

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基金项目(Foundation):

作者(Author): 林华庆,余楚钦,邱五龙
LIN Hua-qing~1,YU Chu-qin~1,QIU Wu-long~2 (1.Institue of Materia Medica

参考文献(References)：

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• [2]中华人民共和国药典委员会.中国药典(2005年版)一部[S].北京:化学工业出版社,2005:94.